Papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens as an adjunct to grouping, antibody screening, or antibody identification procedures.Limited papain digestion has proven useful for structural studies of enzymes and other proteins.3,4 Papain was found to significantly increase the yield of viable smooth muscle cells while not affecting cell sensitivity to stimulants. P4762 ) has been used for the isolation of smooth muscle cells. For example, papain has been used to isolate viable, morphologically intact, cortical neurons from postnatal rats. Papain is commonly used in cell isolation procedures where it has proven more efficient and less destructive than other proteases on certain tissues.It does not accept Val at the P1' position. Papain exhibits a preference for an amino acid bearing a large hydrophobic side chain at the P2 position.
Papain exhibits broad specificity, cleaving peptide bonds of basic amino acids, leucine, or glycine. Papain will digest most protein substrates more extensively than the pancreatic proteases.
